Invitrogen™ M-MLV Reverse Transcriptase反转录酶28025-021
Invitrogen™ M-MLV Reverse Transcriptase反转录酶28025-021
M-MLV (Moloney Murine Leukemia Virus) Reverse Transcriptase (RT) is a recombinant DNA polymerase that synthesizes a
complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. Compared to AMV RT, M-MLV RT lacks DNA endonuclease activity and has a lower RNase H activity.
• Thermostability – Optimal activity at 37°C
• Size of cDNA – MMLV can be used to synthesize first-strand cDNA up to 7 kb
• Applications – Synthesis of first-strand cDNA (1), primer extension (2), sequencing dsDNA (3), cDNA libraries, and RT-PCR (4)
Source: Purified from E. coli expressing the pol gene of M-MLV on a plasmid (5).
Performance and Quality Testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product.
Unit Definition: One unit of M-MLV RT is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly(A)•oligo(dT)
Shipping Condition: | Wet Ice |
Optimal Reaction Temperature: | 37° C |
Final Product Size(s): | 7 kb or less |
Ribonuclease H Activity: | Reduced |
Concentration: | 200 U⁄µl |
Reverse Transcriptase: | M-MLV Reverse Transcriptase |
Form: | Frozen |
Format: | Tube(s) |
Template: | ssRNA, RNA-DNA Hybrid, ssDNA |
Sensitivity: | Low |
Product Size: | 200,000 units |
Enzyme Function: | RNA dependent DNA Polymerase |
Number of Reactions: | 1000 Reactions |
Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |