Invitrogen™ Platinum® PCR SuperMix 11306-016
Platinum® PCR SuperMix 是用于高效 PCR 扩增的 DNA 聚合酶、盐、镁离子和 dNTP 的即用型混合物。 需要做的只是添加模板和引物,设置时间因此而减半。
Platinum® PCR SuperMix 用于高特异性 DNA 扩增 (1)。 重组 Taq DNA 聚合酶和 Taq DNA 聚合酶的 Platinum® 单克隆抗体的混合物提供了高特异性。 自动'热启动'提高了 PCR 的特异性。 在初始变性步骤中活化 Taq DNA 聚合酶。 Platinum® PCR SuperMix: -可在室温下准备反应体系 -可扩增最大达 5 kb 的片段 -生成具有 3'-A 末端的产物 -提高 PCR 特异性 Hot Start: | Built-In Hot Start | Fidelity (vs. Taq): | 1 X | Polymerase: | Platinum® Taq DNA Polymerase | Final Product Size(s): | 5 kb or less | Reaction Format: | SuperMix or Master Mix | Reaction Speed: | Standard | GC-Rich PCR Performance: | Low | Product Size: | 100 reactions | Product Overhang: | 3'-A | Shipping Condition: | Dry Ice | Exonuclease Activity: | 5' - 3' | Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |
Platinum® PCR SuperMix is supplied at 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 5,000 amplification reactions of 50 µl each are provided.
Platinum® PCR SuperMix Components: 22 U/ml complexed recombinant Taq DNA polymerase with Platinum® Taq Antibody, 22 mM Tris-HCl (pH 8.4), 55 mM KCl, 1.65 mM MgCl2, 220 µM dGTP, 220 µM dATP, 220 µM dTTP, 220 µM dCTP, and stabilizers.
100 rxns: 4 x 1.125 ml
Store at -20°C in a non-frost-free freezer. |