Invitrogen™ PCR SuperMix 10572-014
Invitrogen™ PCR SuperMix 10572-014
PCR SuperMix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification.
All you have to do is add template and primers, reducing set-up time by half (Figure 1).
PCR SuperMix contains recombinant Taq DNA polymerase for routine PCR of fragments up to 5kb.
Hot Start: | No |
Fidelity (vs. Taq): | 1 X |
Polymerase: | Taq DNA Polymerase |
Final Product Size(s): | 5 kb or less |
Reaction Format: | SuperMix or Master Mix |
Reaction Speed: | Standard |
GC-Rich PCR Performance: | Low |
Product Size: | 100 reactions |
Product Overhang: | 3'-A |
Exonuclease Activity: | 5' - 3' |
Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |
PCR SuperMix is supplied at 1.1X concentration to allow approximately 10% of the final reaction
volume to be used for the addition of primer and template solutions. Reagents sufficient for 5,000
amplification reactions of 50 ?l each are provided.
PCR SuperMix Components:
22 mM Tris-HCl (pH 8.4), 55 mM KCl, 1.65 mM MgCl2, 220 ?M dGTP, 220 ?M dATP, 220 ?M dTTP, 220 ?M dCTP, 22 U recombinant Taq DNA Polymerase/ml, stabilizers.
100 rxns: 4 × 1.125 ml
Sore at -20°C in a non-frost-free freezer.